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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Rb | IP, ICC, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1317 |
| Description | Anti-SUZ12 Antibody, clone 2AO9 |
| Alternate Names |
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| Background Information | SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates Lys-9 and Lys-27 residues of histone H3. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et Al. (2007). Molecular and Cellular Biology. 27(10):3769–3779. Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et Al. (2007). Molecular and Cellular Biology. 27(10):3769–3779. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human SUZ12. |
| Epitope | Unknown |
| Clone | 2AO9 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes SUZ12. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with rabbit based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq] |
| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates Lys-9 and Lys-27 residues of histone H3. SIZE: 739 amino acids; 83055 Da SUBUNIT: Component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like BMI1, CBX4, CBX8, PHC2, PHC3, SCMH1, RING1 and RNF2. SUBCELLULAR LOCATION: Nucleus (Probable). DISEASE: chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer. SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. & Contains 1 C2H2-type zinc finger. |
| Molecular Weight | ~ 95 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunoprecipitation in HeLa cell lysate. Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |