Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC, WB, IF, IP, IH(P) | M | Affinity Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1410 |
| Replaces | 04-1102 |
| Description | Anti-CD45 Antibody, clone F10-89-4 |
| Alternate Names |
|
| Background Information | CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2a presented in phosphate buffered saline containing 10 mm sodium azide and 1 mg/mL bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD45 Antibody, clone F10-89-4 detects level of CD45 & has been published & validated for use in FC, WB, IF, IP, IH(P). |
| Key Applications |
|
| Application Notes | Immunofluorescence: A previous lot of this antibody was used in the identification of cells of leucocyte origin by indirect staining. Immunohistochemistry (paraffin): A previous lot of this antibody was used in the discrimination between malignant cells of haematopoietic origin and other malignancies in frozen tissue sections. Immunoprecipitation: A previous lot of this antibody was used in studies of the leucocyte common antigen complex and its identification by the Western blotting technique. Flow Cytometry: Studies of CD45 expression and the association with T-cell receptor signaling was performed using this antibody of previous lot. Studies of the mechanism by which CD45 regulates B cell activation through protein tyrosine phospatases. Optimal working dilutions must be determined by the end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of CD45 Monoclonal Antibody: Tonsil |
| Biological Information | |
|---|---|
| Immunogen | Purified T cells from human lymph nodes |
| Clone | F10-89-4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with the leucocyte common antigen (LCA) complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen. The LCA comprises of at least 5 isoforms ranging from 180-220 kDa which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to all of the isoforms. Antigen distribution: Thymocytes > 95% Granulocytes > 95% Monocytes > 95% B cells (CD20+) > 95% T cells (CD3+) > 95% NK cells (CD16+) > 95% Peripheral blood lymphocytes > 95% FUSION PARTNER: NS1 myeloma cell line |
| Isotype | IgG2a |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Expected to react with human and rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. |
| Gene Symbol |
|
| Purification Method | Protein A affinity chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN. SIZE: 1304 amino acids; 147254 Da SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1. PTM: Heavily N- and O-glycosylated. DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains. |
| Molecular Weight | ~180-220 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on jurkat cell lysate. Western Blot Analysis: 1:500 dilution of this lot detected CD45 on 10 μg of Jurkat lysates |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |