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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1416 |
| Replaces | CBL1326 |
| Description | Anti-CD45 Antibody, clone IBL-5/25 |
| Alternate Names |
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| Background Information | CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG in phosphate buffered saline containing 10 mm sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD45 Antibody, clone IBL-5/25 is an antibody against CD45 for use in FC, IH. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A previous lot detected CD45, clone IBL-5/25 in mouse peripheral blood lymphocytes |
| Biological Information | |
|---|---|
| Immunogen | Mouse lymph node cells. |
| Clone | IBL-5/25 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | This antibody reacts with all isoforms of mouse CD45 which is expressed on all haematopoietic cells except erythrocytes. FUSION PARTNER: SP2/0 myeloma cell line |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with mouse. Reactivity with other species has not been determined. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | SUBUNIT STRUCTURE: Interacts with CD45/PTPRC. SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein Potential. PTM: Phosphorylated on tyrosine residues. |
| Molecular Weight | Approx. 147 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse colon tissue. Immunohistochemistry Analysis: 1:100 dilution of this antibody detected CD45 in mouse colon tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8°C for 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |