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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1478 |
| Description | Anti-RUNX2 Antibody, clone AS110 |
| Alternate Names |
|
| Background Information | RUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers. They include LCK, T-cell receptor enhancers, and various bone related genes such as osteocalcin, osteopontin, and bone sialoprotein. As a result of this, RUNX2 is involved in osteoblastic differentiation and skeletal morphogenesis. RUNX2 contains a proline/serine/ threonine-rich region at its C-terminus that has shown to be critical for its transcriptional activity. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RUNX2 Antibody, clone AS110 detects level of RUNX2 & has been published & validated for use in WB & IC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-coupled recombinant protein corresponding to amino acids 311-415 of human RUNX2. |
| Epitope | Internal |
| Clone | AS110 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Detects RUNX2 |
| Isotype | IgG2b |
| Species Reactivity |
|
| Species Reactivity Note | Human. Other species have not been tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. Inhibits MYST4-dependent transcriptional activation. SUBUNIT: Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 By similarity. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4. SUBCELLULAR LOCATION: Nucleus. SPECIFICITY: Specifically expressed in osteoblasts. DOMAIN: A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. PTM: Phosphorylated; probably by MAP kinases (MAPK) By similarity. Isoform 3 is phosphorylated on Ser-340. DISEASE: Defects in RUNX2 are the cause of cleidocranial dysplasia (CCD) [MIM:119600]. CCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. Ref.1 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 SIMILARITY:Contains 1 Runt domain. |
| Molecular Weight | 54-57 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot. Western Blot Analysis: Anti-RUNX2, clone AS110 detected RUNX at 1:1,000 to 1:2,000 dilution in HL-60 cell lysate resolved via SDS-PAGE and transferred to PVDF. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |