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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1529 |
| Description | Anti-FXR1 Antibody, clone 6BG10 |
| Alternate Names |
|
| Background Information | Fragile X mental retardation-related protein 1 (FXR1) and FXR2 are members of the FMR1 family sharing a very high degree of protein similarity among them. They interact with each other and also with FMR1, moving between the cytoplasm and the nucleus. FXR1 is an RNA binding protein and is expressed in the heart, kidney, brain, and testis. Mutations in FMR1 result in the most common form of mental retardation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-FXR1 Antibody, clone 6BG10 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect FXR1 also known as fragile X mental retardation autosomal homolog 1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FXR1 in HeLa and NIH/3T3 cell lysates. Immunoprecipitation Analysis: A representative lot was used in an independent laboratory in immunoprecipitation. Siomi, M., et Al. (1996). Molecular and Cellular Biology. 3825-3832. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human FXR1. |
| Epitope | Unknown |
| Clone | 6BG10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes FXR1. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs By similarity. SUBUNIT STRUCTURE: Interacts with FMR1 and FXR2. Interacts with TDRD3, and with CYFIP2 but not with CYFIP1. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICTY: Expressed in all tissues examined including heart, brain, kidney and testis. PTM: Arg-445 is dimethylated, probably to asymmetric dimethylarginine. SEQUENCE SIMILARITIES: Belongs to the FMR1 family. Contains 2 KH domains. |
| Molecular Weight | 68 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected FXR1 on 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |