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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1583 |
| Description | Anti-PRL-2 Antibody, clone 42 |
| Alternate Names |
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| Background Information | PRL-2 is one of three members (PRL-1, PRL-2, and PRL-3) that is part of a unique protein-tyrosine phosphatase ((PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP is known to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferres a transformed phenotype and can result in a tumor growth in nude mice, suggesting a role in tumorigenesis. Alternatively spliced transcript variants that encode two distinct isoforms have been described |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PRL-2 Antibody, clone 42 detects level of PRL-2 & has been published & validated for use in WB, IH & IP. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse PRL-2. |
| Epitope | Unknown |
| Clone | 42 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes PRL-2. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyltransferase type II activity by blocking the association between RABGGTA and RABGGTB By similarity. CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. ENZYME REGULATION: Inhibited by sodium orthovanadate and pentamidine By similarity. SUBUNIT STRUCTURE: In contrast to PTP4A1 and PTP4A3, does not interact with tubulin. Interacts with RABGGTB By similarity. SUBCELLULAR LOCATION: Cell membrane. Early endosome. Cytoplasm TISSUE SPECIFICTY: Expressed in skeletal muscle, and at lower levels in liver, lung, heart, kidney, brain, testis and spleen. Ref.1 PTM: Farnesylated. Farnesylation is required for membrane targeting and for interaction with RABGGTB By similarity. SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain. |
| Molecular Weight | ~ 19 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected PRL-2 on 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |