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Millipore Anti-Foxo3a/Fkhrl1 Antibody, Clone 15f7.2 Is A Mouse Monoclonal Antibody For Detection Of Foxo3a/Fkhrl1 Also Known As Forkhead Box Protein O3, Af6q21 Protein, Forkhead In - Mill (Additional S&H or Hazmat Fees May Apply)
Forkhead box protein O3 (FOXO3a), or FKHRL1, is a ubiquitous transcription factor that triggers the expression of pro-apoptotic genes under conditions of oxidative stress. FOXO3a is regulated by the Akt kinase which phosphorylates FOXO3a on serine 253, 315, and on threonine 32 residues. Modified FOXO3a is recognized by the 14-3-3 proteins which export FOXO3a to the cytoplasm, and consequently terminates its transcriptional activity. FOXO3a may also be regulated by lysine methylation in neuronal cells.
Product Information
Format
Purified
Control
Rat liver tissue lysate.
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-FOXO3a/FKHRL1 Antibody, clone 15F7.2 is a Mouse Monoclonal Antibody for detection of FOXO3a/FKHRL1 also known as Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1 & has been validated in WB & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected FOXO3a/FKHRL1 in human thyroid gland tissue and human breast cancer tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human FOX03a.
Clone
15F7.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001446
Gene Symbol
FOXO3
FKHRL1
FOXO3A
Purification Method
Protein G Purified
UniProt Number
O43524
Molecular Weight
~71 kDa observed. An uncharacterized band may be observed at ~28 kDa in some tissue lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in rat liver tissue lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected FOXO3a/FKHRL1 in 10 mg of rat liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
