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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IHC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-354 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Clusterin α chain (human) Antibody, clone 41D |
| Alternate Names |
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| Background Information | Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Clusterin α chain (human) Antibody, clone 41D is an antibody against Clusterin α chain (human) for use in IC, IH, IH(P) & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections. |
| Biological Information | |
|---|---|
| Immunogen | Native clusterin purified from human serum. Clone 41D. |
| Clone | 41D |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P10909 # Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death (apoptosis). SIZE: 449 amino acids; 52495 Da SUBUNIT: Antiparallel disulfide-linked heterodimer. Interacts with CLUAP1. SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P10909 ## Belongs to the clusterin family. |
| Molecular Weight | 40 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates. Western Blot Analysis: 0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |