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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | ICC | M | AlexaFluor®647 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-591-AF647 |
| Description | Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 647 |
| Alternate Names |
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| Background Information | RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1; UniProt P31749; also known as PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID 207) in human. AKTs (protein kinase Bs) are serine/threonine kinases that play an important role in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. There exit three types of AKTs (α, β, γ or AKT 1, 2, 3) sharing a high degree of homology, but differing slightly in the localization of their regulatory phosphorylation sites. AKT α is the predominant isoform in most tissues, whereas the highest expression of AKT β is observed in the insulin-responsive tissues, and AKT γ is abundant in brain tissue. Each AKT type is composed of three functionally distinct regions, including an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif. |
| Product Information | |
|---|---|
| Format | AlexaFluor®647 |
| Presentation | Purified mouse monoclonal IgG antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Akt Antibody, PH Domain, clone SKB1, Alexa Fluor® 647 is validated for use in Immunocytochemistry for the detection of PKB. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Akt/PKB in A431 cells. The unconjugated antibody (Cat. No. 05-591) is shown to be suitable also for flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications. |
| Biological Information | |
|---|---|
| Immunogen | GST-fusion protein corresponding to residues 1-149 of human Akt1. |
| Epitope | Amino acids 1-149 |
| Clone | SKB1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone SKB1 recognizes Akt1 and Akt2, but not Akt3, GST fusion proteins at 100 ng by immunoblotting analysis using the unconjugated antibody (Cat. No. 05-591). |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Human, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~60 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Akt/PKB in HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |