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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Rb, Ca | FC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-717 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2 |
| Alternate Names |
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| Background Information | Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2 is an antibody against Adenosine Receptor A2a clone 7F6-G5-A2 for use in FC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1 μg/mL of a previous lot detected Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice. FACS Analysis: A previous lot of this antibody has been reported by an independent laboratory to detect Adenosine Receptor A2A in transfected HEK293 cells (Koshiba, M., 1999). |
| Biological Information | |
|---|---|
| Immunogen | 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. Clone 7F6-G5-A2. |
| Clone | 7F6-G5-A2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes adenosine receptor A2A, Mr 43 kDa. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, rat, rabbit, and dog. Cross-reactivity with guinea pig also reported. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P29274 # Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. SIZE: 412 amino acids; 44707 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P29274 ## Belongs to the G-protein coupled receptor 1 family. |
| Molecular Weight | ~43 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunohistochemistry. Western Blot Analysis: A 1:1000–1:5000 dilution of this lot of antibody detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras (Rosin, D.L., 1998). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |