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Millipore This Anti-Α-Tubulin Antibody, Clone Dm1a, Alexa Fluor® 555 Conjugate Is Validated For Use In Immunocytochemistry For The Detection Of Α-Tubulin. - Mill (Additional S&H or Hazmat Fees May Apply)
Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tubes. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.
Product Information
Format
AlexaFluor®555
Presentation
Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of α-Tubulin.
Key Applications
Immunocytochemistry
Application Notes
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Biological Information
Immunogen
Purified chicken brain α-tubulin.
Epitope
C-terminal 25 amino acids.
Clone
DM1A
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).
Isotype
IgG1
Species Reactivity
Mouse
Human
Guinea Pig
Avian
Bovine
Porcine
Gerbil
Rat
Species Reactivity Note
Mouse, Human, Guinea Pig, Avian, Bovine, Porcine, Gerbil, Rat.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001292201.1
Gene Symbol
TUBA1C
TUBA1
Purification Method
Protein g purified.
UniProt Number
P02552
Molecular Weight
~50 kDa observed.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-tubulin in A431 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
