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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M | WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-957-I |
| Description | Anti-ERK1/MAPK3 Antibody, CT Antibody, clone UP-158-22-3-10 |
| Alternate Names |
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| Background Information | Mitogen-activated protein kinase 3 (EC 2.7.11.24; UniProt P21708; also known as ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, MNK1, p44-ERK1, p44-MAPK) is encoded by the Mapk3 (also known as Erk1, Prkm3) gene (Gene ID 50689) in rat species. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and -Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Rabbit monoclonal antibody IgG hybridoma culture supernatant without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit monoclonal Anti-ERK1/MAPK3 Antibody, CT, clone UP-158-22-3-10, Cat. No. 05-957-I detects levels of ERK1/MAPK3, and has been published and validated for use in Immunoprecipitation and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated active Erk1 from lysate of NGF-stimulated (50 ng/mL) PC-12 rat pheochromocytoma cells. Western Blotting Analysis: A representative lot detected Erk1 in human MCF-7 is a breast cancer cell lysate (Chen, J.Q., et al. (2013). Anal. Biochem. 442(1):97-103). Western Blotting Analysis: A representative lot detected Erk1 in human HaCat keratinocyte lysate (Jarosz, M., et al. (2012). PLoS One. 7(6):e39436). Western Blotting Analysis: A representative lot detected Erk1 in HT-29 human colorectal adenocarcinoma cell lysate (ONeill, R.A., et al. (2006). Proc. Natl. Acad. Sci. U. S. A. 103(44):16153-16158). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat ERK-1 (MAPK 3). |
| Clone | UP-158-22-3-10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Clone UP-158-22-3-10 recognizes a C-terminal epitope of MAPK 3 (ERK1). Immunogen sequence is not present in human MAPK3 spliced isoform 3 (ERK1b). |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified. |
| UniProt Number |
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| Molecular Weight | ~44 kDa observed. 43.00/38.14 kDa (human MAPK3 isoform 1/2), 42.95/45.64 kDa (rat MAPK3 isoform 1/2), 42.93 kDa (mouse MAPK3) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this hybridoma culture supernatant detected Erk1 in 10 µg of mouse NIH/3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |