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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1085-I |
| Description | Anti-IL-7Ra (CD127), extracellular Antibody |
| Alternate Names |
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| Background Information | The R34-34 antibody is specific for human CD127, a cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity. The R34-34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM), whereas certain populations of Foxp3+ /CD25+ T cells lacking CD127 have been associated with impaired suppressive function (Bikker et al. 2012. Rheumatology, 51: 996-1005) |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-IL-7Ra (CD127), extracellular Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of IL-7Ra (CD127), extracellular. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2.0 µg/mL from a representative lot detected IL-7Ra (CD127) in 10 µg of human spleen tissue lysate. Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected IL-7Ra (CD127) in 1X10E6 human peripheral blood mononuclear cells (PBMCs). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the extracellular domain of human IL-7Ra (CD127). |
| Epitope | Extracellular domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~70 kDa observed. This protien is highly and variably glycosylated, and may vary from the calculated molecular weight of ~52kDa (Vranjkovi, A., et al., International Immunology, Vol. 19, No. 12, pp. 1329–1339). Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-7Ra (CD127) in 10 µg of K562 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |