Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1304 |
| Replaces | 07-316 |
| Description | Anti-SETD8 (hPR-SET7) Antibody |
| Alternate Names |
|
| Background Information | N-lysine methyltransferase KMT5A (UniProt: Q9NQR1; also known as EC: 2.1.1.43, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8) is encoded by the KMT5A (also known as PRSET7, SET07, SET8, SETD8) gene (Gene ID: 387893) in human. SETD8 (hPR-SET7) is a histone methyltransferase that adds a single methyl group to lysine 20 of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. SETD8 is not detected during the G1 phase. It is first detected during S through G2 phases, and peaks during mitosis. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis and is also involved in chromosome condensation and proper cytokinesis. It is also reported to mediate mono-methylation of p53/TP53 at lysine 382, leading to repress p53/TP53-target genes. SETD8 has a SET domain in the amino acids 257-378 and this region contains the active site of enzymatic activity. Sequences upstream and downstream of SET domain are also required for its methyltransferase activity. Two isoforms of SETD8 have been described that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mm NaCl and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SETD8 (hPR-SET7) Antibody is a Rabbit Polyclonal Antibody for detection of SETD8 (hPR-SET7) also known as Histone-lysine N-methyltransferase SETD8 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human SETD8 (hPR-SET7). |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects N-lysine methyltransferase KMT5A (PR-SET7). |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human. Predicted to react with mouse and rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | Function: Histone methyltransferase that specifically monomethylates Lys-20 of histone H4. H4 Lys-20 monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Catalytic activity: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Subcellular location: Nucleus. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin. Developmental stage: Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level). Induction: By HCFC1 C-terminal chain, independently of HCFC1 N-terminal chain. Domain: Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity. Sequence similarities: Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Contains 1 SET domain. |
| Molecular Weight | ~43 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell nuclear extract. Western Blotting Analysis: 0.05 µg/mL of this antibody detected SETD8 (hPR-SET7) in HeLa cell nucleaar extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at 2-8°C for 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |