Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, FC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-1328 |
| Description | Anti-Themis Antibody |
| Alternate Names |
|
| Background Information | Protein Themis (UniProt: Q8BGW0; also known as Grb2-associating protein, Gasp, Protein Thylex, Thymocyte-expressed molecule involved in selection) is encoded by the Themis gene (Gene ID: 210757) in murine species. T cell development in the thymus is shown to follow an ordered progression from CD4- 8- (double negative, DN) through CD4+8+ (double positive, DP) to single positive (SP, CD4+8 or CD8+4 ) stages, which depends on a complex interplay of signaling pathways. Themis plays an important role in late thymocyte development by controlling both positive and negative T-cell selection. It is required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Themis is expressed in the thymus and to a lesser extent in the spleen but is not detectable in non-lymphoid tissues. It is highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in mature T-cells. Mice lacking Themis display a defect in the selection process mediated by the positive selection checkpoints, resulting in reduced numbers of SP cells and mature peripheral T cells. Themis contains two novel cysteine-based CABIT (cysteine-containing, all beta in Themis) domains (aa 1-260 and 261-520), a bipartite type nuclear localization sequence and a proline-rich sequence. CABIT domain modulate Themis by interacting directly with the PTP domain of SHP-1 and stabilize the catalytic cysteine residue of SHP-1, leading to inhibition of its tyrosine phosphatase activity. (Ref.: Espinoza, JA., et Al. (2017). Cell. Mol. Immunol. 14; 721-723; Fu, g., et Al. (2009). Nat. Immunol. 10(8); 848-856). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Themis, Cat. No. 06-1328, is a rabbit monoclonal antibody that detects Themis and is tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting. for the detection of Themis. |
| Key Applications |
|
| Application Notes | Tested Applications Immunopreciptiation Analysis: A representative lot of this antibody was used to immunoprecipitate Themis in wild-type thymocytes, but not in Themis knockout thymocytes (Fu, g., et Al. (2013) Nature. 504(7480); 441-445. Flow Cytometry Analysis: A representative lot of this antibody used by an independent laboratory in Flow Cytometry. (Vasily Rybakin, pH.D., Gascoigne lab, Scripps Research Institute, La Jolla, CA) Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of Mouse Themis. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Thymis protein. It targets an epitope within 13 amino acids from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. SUBUNIT STRUCTURE: Interacts with PLCG1, ITK and GRB2. SUBCELLULAR LOCATION: Cytoplasm. Nucleus TISSUE SPECIFICTY: Expressed in the thymus and to a lesser extent in the spleen but not detectable in nonlymphoid tissues. Highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in mature T-cells (at protein level). INDUCTION: Down-regulated by stimulation through the alpha-beta TCR. PTM: Phosphorylated on Tyr residues quickly after TCR stimulation. DISRUPTION: phenotype Mice are viable, are born at the expected mendelian frequency and present no gross abnormalities. They however display a blockade in late T-cell development with defects in thymocyte selection. The number of transitional CD4+CD8(int) thymocytes as well as CD4+ or CD8+ single-positive thymocytes is lower. Thymocytes show defective positive selection, resulting in fewer mature thymocytes. Negative selection is also impaired. A greater percentage of T-cells have CD4+CD25+Foxp3+ regulatory and CD62L(lo)CD44(hi) memory phenotypes compared to wild-type T-cells. SEQUENCE SIMILARITIES: Belongs to the themis family. |
| Molecular Weight | Target molecular weight ~75 kDa observed; 72.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Mouse Thymus tissue lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Themis in Mouse Thymus tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |