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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | WB, IH(P), IP, ChIP, Gel Electrophoresis | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-519 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-phospho-CREB (Ser133) Antibody |
| Alternate Names |
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| Background Information | CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected phospho CREB (Ser133) in rat cerebral cortex, human cerebral cortex, and rat hippocampus tissue. Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho CREB (Ser133) in 500 µg of BALB/3T3 A31+Forskolin cell lysate. Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho CREB (Ser133) in 4 µg of HEK293T cell lysate. Gel Shift (EMSA): This antibody has been shown to gel shift by independent labs. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the KID of human phospho CREB (Ser133). |
| Epitope | phospho-Ser133 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Human, Rat. Predicted to react with Bovine, Canine, Porcine, Xenopus, Zebrafish based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column. |
| UniProt Number |
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| Molecular Weight | ~43 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Forskolin treated BALB/3T3 clone A31 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho CREB (Ser133) in 10 µg of Forskolin treated BALB/3T3 clone A31 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |