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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC | Rb | AlexaFluor®555 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-570-AF555 |
| Description | Anti-phospho-H3 Antibody (Ser10), Alexa Fluor® 555 Conjugate |
| Alternate Names |
|
| Background Information | Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 15 mg/mL BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate is a purified rabbit polyclonal Antibody that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 555 and validated for use in IC. The unconjugated version of this antibody is published & proven in ICC, IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) immunoprecipitated phospho-Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate. Beadlyte® Assay Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 06-570) recognized Histone H3 phosphorylated on Ser10 by Luminex assay. Western Blot Analysis: 0.5-1 µg/mL from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) detected phospho-Histone H3 (Ser10) on 10 µg of Colcemid-treated HeLa acid extract, but not on untreated HeLa acid extract. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to phospho-Histone H3 (Ser10). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |