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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ICC, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-600 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-NR2B Antibody |
| Alternate Names |
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| Background Information | NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimers disease, epilepsy and ischemic neuronal cell death. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, and 0.05% sodium azide. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect NR2B using this Anti-NR2B Antibody validated for use in IC, IP & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | a.a. 1437-1456 of mature mouse NR2B or 1463-1482 of NR2B precursor; signal is a.a. residues 1-26 [KFNGSSNG HVYEKLSSIESDV]. This sequence is identical to a.a. 1437-1456 of mature rat NR2B and a.a. 1465-1484 of human NR3, containing a N-terminal lysine. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | NR2B; the immungen sequence is identical in mouse, rat, human, bovine, dog and chimp NR2B. Human NR2B is sometimes referred to as hNR3 in the literature. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse (M). Predicted to react with Bovine (B), Canine (Ca), Human (H), Monkey (Mk), Rat (R), Chimpanzee (Chp) based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q13224 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. SIZE: 1484 amino acids; 166367 Da SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL and DLG4 (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. SIMILARITY: SwissProt: Q13224 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family. |
| Molecular Weight | 180 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on rat brain microsomal preparation (Catalog # 12-144). Western Blot Analysis: 0.5-2 μg/mL of this lot of antibody detected NR2B (~180 kDa) in rat brain microsomal preparation (Catalog # 12-144). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Aliquot to avoid freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 250 µg |