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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, M, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-820 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-Adducin (Ser662) Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho-Adducin (Ser662) Antibody is validated for use in WB for the detection of phospho-Adducin (Ser662). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide (C-KKFRTPpSFLKKNK) corresponding to amino acids 656-668 of human gamma-Adducin |
| Host | Rabbit |
| Specificity | phosphorylated serine 662 of γ-Adducin, phosphorylated serine 724 of α-Adducin, phosphorylated serine 713 of β-Adducin |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. |
| Gene Symbol |
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| Modifications |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9UEY8 # Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. SIZE: 706 amino acids; 79155 Da SUBUNIT: Heterodimer of an alpha and a gamma subunit. TISSUE SPECIFICITY: Heart only expresses isoform 1. DOMAIN:SwissProt: Q9UEY8 Comprised of three regions: a N-terminal protease- resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. PTM: Sumoylated. SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily. |
| Molecular Weight | 80kDa, 120kDa, 110kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells or on canine MDCK cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |