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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-864 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-FLIP Antibody, CT |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | PBS, pH 7.4, 0.02% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect FLIP using this Anti-FLIP Antibody, CT validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide corresponding to amino acids 447-464 [NGYMYDWNSRVSAKEKYY] of human FLIP |
| Host | Rabbit |
| Specificity | long form of FLIP |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O15519 # Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. SIZE: 480 amino acids; 55344 Da SUBUNIT: TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. TISSUE SPECIFICITY: Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. DOMAIN: SwissProt: O15519 The caspase domain lacks the active sites residues involved in catalysis. PTM: Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. SIMILARITY: Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains. |
| Molecular Weight | 55kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on Jurkat, A431, Raji, 3T3, CTLL, PC12 or L6 RIPA cell lysates |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |