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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | WB, IH(P), IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-868 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-GABAA Receptor α1 Antibody |
| Alternate Names |
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| Background Information | The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-GABAA Receptor α1 Antibody is validated for use in IH, IP, WB for the detection of GABAA Receptor α1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABAA Receptor α1 in rat hippocampus tissue. Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated GABAA Receptor α1 in rat brain microsomal preparation RIPA lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the topological domain of rat GABAA Receptor α1. |
| Epitope | Topological domain. |
| Host | Rabbit |
| Specificity | This antibody recognizes the topographical domain of GABAA Receptor α1. |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with Rat and Mouse. Other homologies: Human (94% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Subunit of GABAA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain. |
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. SUBUNIT STRUCTURE: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1. Interacts with TRAK1. SUBCELLULAR LOCATION: Cell junction > synapse > postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| Molecular Weight | ~51 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in rat brain microsomal preparation tissue lysate. Western Blot Analysis: A 1:10,000 dilution of this antibody detected GABAA Receptor α1 in 10 µg of rat brain microsomal preparation tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |