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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-049 |
| Brand Family | Upstate |
| Description | Anti-Nedd4 |
| Alternate Names |
|
| Background Information | E3 ubiquitin-protein ligase NEDD4 (UniProt: P46934; also known as EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase Nedd4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4) is encoded by the Nedd4 (also known as KIAA0093, Nedd4-1) gene (Gene ID: 4734) in human. NEDD4 is a peripheral membrane protein that is recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, it follows IGF1R internalization and remains associated with early endosomes. Nedd4 can accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. It is shown to specifically ubiquitinate Lysine 63 in target proteins. It also monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. NEDD4 is reported to polyubiquitinate the epithelial sodium channel and any mutations that disrupt association between Nedd4 and sodium channel can lead to Liddle s syndrome, a hyperaldosteronism-like hypertension disease. NEDD4 can interact with viral proteins that contain a late-budding motif P-P-P-Y and this interaction is believed to be essential for viral particle budding of many retroviruses, like HTLV-1 Gag, and MLV Gag. It contains four WW domains (WW 1-4; aa 610-643; 767-800; 840-873; and 892-925) and one HECT domain (aa 984-1318). The WW domains mediate its interaction with PPxY motif-containing proteins. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nedd4 Antibody, Cat. No. 07-049, is a rabbit polyclonal antibody that detects Nedd4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected NEDD44 in rat cerebral cortex, rat cerebellum, and human colon cancer tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 68 amino acids from the N-terminal half of human Nedd4. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects E3 ubiquitin-protein ligase Nedd4. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P46934 # E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Involved in the budding of many viruses. SIZE: 1000 amino acids; 114936 Da SUBUNIT: Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2 (By similarity). Interacts with viral proteins that contain a late- budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag. SUBCELLULAR LOCATION: Cytoplasm (By similarity). DOMAIN: SwissProt: P46934 The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2 (By similarity). SIMILARITY: Contains 1 C2 domain. & Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. & Contains 4 WW domains. MISCELLANEOUS: A cysteine residue is required for ubiquitin- thioester formation. |
| Molecular Weight | ~110 kDa observed; 102.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat kidney tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |