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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, Ca, R | IH(P), WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1213 |
| Replaces | AB9072 |
| Description | Anti-IP3 Receptor 1 Antibody |
| Alternate Names |
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| Background Information | IP3 Receptor 1 is an intracellular channel that when stimulated by inositol 1,4,5-triphosphate mediates the release of calcium from the endoplasmic reticulum. Defects within the gene ITPR1 cause a group of cerebellar disorders known as spinocerebellar ataxia type 15 (SCA15). This receptor is widely expressed in tissue. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
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| Presentation | Rabbit serum. Liquid |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-IP3 Receptor 1 Antibody is validated for use in IH(P), WB for the detection of IP3 Receptor 1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected IP3 Receptor 1 in mouse cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from the N-terminal of human IP3 Receptor 1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody reacts with the 300 kDa IP3 Receptor 1 protein. |
| Isotype | mixed IgGs |
| Species Reactivity |
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| Species Reactivity Note | Proven to react with mouse and human. Expected to react with canine and rat based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified rabbit serum |
| UniProt Number |
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| UniProt Summary | FUNCTION: Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. SUBUNIT STRUCTURE: Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with AHCYL1 By similarity. Interacts with MRVI1 and CABP1 (via N-terminus). SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed. DOMAIN: The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. PTM: Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels By similarity. Phosphorylated on tyrosine residues. INVOLVEMENT IN DISEASE: Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory. MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor. SEQUENCE SIMILARITIES: Belongs to the InsP3 receptor family. Contains 5 MIR domains. |
| Molecular Weight | Approx. 300 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in C2C12 cell lysates. Western Blot Analysis: 1:500 dilution of this lot detected IP3 RECPT 1 on 10 ug of C2C12 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 1 year date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |