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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1223 |
| Replaces | AB4090 |
| Description | Anti-Protein Phosphatase 2 C α/β Antibody |
| Alternate Names |
|
| Background Information | PP-2 C α/β is heterodimer located in the mitochondrial matrix. Within the pyruvate dehydrogenase complex, PP-2 C α/β serves to catalyze the dephosphorylation and concomitant reactivation of the E1 component’s alpha subunit. Mutations occurring in the PDP1 gene are the cause of a disease known as pyruvate dehydrogenase deficiency (PDP deficiency), which leads to neurological dysfunction from lactic acidosis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified immunoglobulin in phosphate buffered saline with 0.08% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Protein Phosphatase 2 C α/β using this Anti-Protein Phosphatase 2 C α/β Antibody validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Purified peptide conjugated to KLH. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes the alpha/beta regulatory subunit isotype of protein phosphatase 2C (PP2C). PP 2C alpha/beta polyclonal antibody binds a 44-46 kDa protein. |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human and rat. Expected to react with mouse based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9P0J1 # Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex (By similarity). COFACTOR: Binds 2 magnesium ions per subunit (By similarity). SIZE: 537 amino acids; 61054 Da SUBUNIT: Heterodimer of a catalytic subunit and a FAD protein of unknown function (By similarity). SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity). DISEASE:SwissProt: Q9P0J1 # Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency) [MIM:608782]. PDP deficiency results in lactic acidosis leading to neurological dysfunction. SIMILARITY: SwissProt: Q9P0J1 ## Belongs to the PP2C family. |
| Molecular Weight | Approx. 46 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in L6 cell lysates. Western Blot Analysis: 1:500 dilution of this lot detected PP-2 C ALPHA/BETA on 10 ug of L6 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |