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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1348 |
| Description | Anti-phospho-PLK1 (Ser137) Antibody |
| Alternate Names |
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| Background Information | Polo-like Kinase 1 (PLK1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-PLK1 (Ser137) using this Anti-phospho-PLK1 (Ser137) Antibody validated for use in WB. |
| Key Applications |
|
| Application Notes | Western Blot (SNAP ID) Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in human placenta tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human phosphoylated PLK1 at and around Ser137. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes phosphorylated PLK1 at Ser137. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat and Mouse. Predicted to react with Cow based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Activated by serine and threonine phosphorylation. SUBUNIT STRUCTURE: Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A. Interacts with BTBD12/SLX4. Ref.11 Ref.12 Ref.13 Ref.14 Ref.18 SUBCELLULAR LOCATION:Nucleus. TISSUE SPECIFICITY:Placenta and colon. DEVELOPMENTAL STAGE:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. INDUCTION: By growth-stimulating agents. PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 2 POLO box domains. Contains 1 protein kinase domain. |
| Molecular Weight | 68 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human placenta tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in lambda phosphatase treated and untreated human placenta tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |