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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1364 |
| Description | Anti-ECT2 Antibody |
| Alternate Names |
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| Background Information | Protein ECT2 (UniProt: Q9H8V3; also known as Epithelial cell-transforming sequence 2 oncogene) is encoded by the ECT2 gene (Gene ID: 1894) in human. ECT2 is a Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP and promotes guanine nucleotide exchange on the Rho family members of small GTPases. It is expressed in lung epithelial cells. Its expression is also observed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. Its levels are up-regulated by calcium in cells forming cell-cell contact sites. It is also up-regulated by DNA damaging agents like hydrogen peroxide and ionizing radiation. ECT2 is required for signal transduction pathways involved in the regulation of cytokinesis. It plays a role in the control of mitotic spindle assembly and regulates the activation of Cdc42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. It contains two BCRT domains (aa 171-260 and 266-354) and the second BCRT domain is involved in inhibition, probably by helping to hamper RhoA binding. ECT2 is autoinhibited by the C-terminal PH domain (aa 675-794) that folds back and binds to the surface of the DH domain (aa 452-641), blocking binding of RhoA to the catalytic center of the DH domain. ECT2 is phosphorylated by PLK1 in vitro and is hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at threonine 373 occurs during the G2/M phase that relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at threonine 444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. (Ref.: Chen, M., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(2); 1027-1035; Kim, JE., et al. (2005). J. Biol. Chem. 280(7); 5733-5739; Tatsumoto, T., et al. (1999). J. Cell Biol. 147(5); 921-928). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ECT2, Cat. No. 07-1364, is a rabbit polyclonal antibody that detects ECT2 protein and is tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Protein ECT2 in A549 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human ECT2. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects ECT2. It targets an epitope within 15 amino acids from the C-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Mouse, Monkey, Bovine, Opossum, Porcine, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Binds highly specifically to RhoA, RhoC and Rac proteins, but does not appear to catalyze guanine nucleotide exchange By similarity. SUBUNIT STRUCTURE: Associates with RACGAP1 at anaphase and during cytokinesis. SEQUENCE SIMILARITES: Contains 2 BRCT domains. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. CAUTION: It is uncertain whether Met-1 or Met-146 is the initiator. |
| Molecular Weight | ~105 kDa observed; 103.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A549 cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Protein ECT2 in A549 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |