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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1401-AF488 |
| Description | Anti-GLUT-1 Antibody, CT, Alexa Fluor® 488 Conjugate |
| Alternate Names |
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| Background Information | Solute carrier family 2, facilitated glucose transporter member 1 (UniProt P11166; also known as Glucose transporter type 1 erythrocyte/brain, GLUT-1, HepG2 glucose transporter, Human T-cell leukemia virus I and II receptor, Receptor for HTLV-1 and HTLV-2) is encoded by the SLC2A1 (also known as DYT9, DYT17, DYT18, EIG12, GLUT, GLUT1, HTLVR, GLUT1DS, PED) gene (Gene ID 6513) in human. Glucose transporters (GLUT-1 to GLUT-7) constitute a family of integral membrane glycoproteins that facilitate cellular glucose uptake. In addition to mediateing glucose uptake in adult tissues, GLUT-1 is the predominant glucose transporter in embryonic and fetal tissues. Two forms of GLUT-1 are found in human frontal cortex, the 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified rabbit conjugate antibody in PBS with 15 mg/mL BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-GLUT-1 Antibody, CT, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of GLUT-1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GLUT-1 in HeLa and NIH/3T3 cells. The unconjugated antibody (Cat. No. 07-1401) is shown to be suitable also for ELISA, Western blotting, immunocytochemistry, and immunohistochemistry applications. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV). |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody recognizes GLUT-1. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Feline, Bovine, Porcine, Equine, Monkey based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~54 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GLUT-1 in A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |