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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1431 |
| Replaces | AB3126 |
| Description | Anti-Tie-2 Antibody, NT |
| Alternate Names |
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| Background Information | Tie-2 is a member of the tyrosine protein kinase family, is a transmembrane receptor for angiopoietin 1, and may represent one of the earliest markers for cell lineage within mammalian endothelial cells. Its expression is almost only found in human, mouse and rat endothelial cells and angioblasts. Most likely, Tie-2 regulates the differentiation, proper dispersal, and proliferation of these cells during angiogenesis. Mutations in TEK are a cause of VMCM or dominantly inherited venous malformations which are characterized by serpiginious, dilated veins. Tie-2 can be found in placenta and lung tissue. Lower levels of the protein can also be found in kidney, umbilical vein endothelial cells, and brain. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Affinity purified immunoglobulin. Liquid in 100 mm Tris, pH 7.5, containing 0.2% BSA and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tie-2 Antibody, N-terminus is validated for use in WB for the detection of Tie-2. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | 18 amino acid peptide sequence within the extracellular, N-terminus of human Tie-2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes human Tunica internal endothelial cell kinase (TIE-2), also described as Angiopoietin-1 receptor, p140 TEK, or tyrosine protein kinase receptor. The peptide sequence used as immunogen is 100% conserved in human, rat, mouse, and 94% in bovine. |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Interacts with PTPRB. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney. PTM: Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB. INVOLVEMENT IN DISEASE: Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. Contains 3 EGF-like domains. Contains 3 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain. |
| Molecular Weight | Approx. 126 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected Tie-2 in 10 µg of human brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |