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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1441 |
| Replaces | AB3793 |
| Description | Anti-phospho-Paxillin (Ser126) Antibody |
| Alternate Names |
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| Background Information | Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | PBS (no Ca2+, no Mg2+), pH 7.3, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-Paxillin (Ser126) Antibody is an antibody against phospho-Paxillin (Ser126) for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugagted synthetic peptide corresponding to human Paxillin at phosphorylated Ser126. |
| Epitope | Ser126 |
| Host | Rabbit |
| Specificity | This antibody recognizes Paxillin phosphorylated at Ser126. |
| Species Reactivity |
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| Species Reactivity Note | Proven to react with human and mouse. Predicted to react with rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION:: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). SUBUNITY STRUCTURE: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 By similarity. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. PTM:Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. SEQUENCE SIMILARITIES: Belongs to the paxillin family. Contains 4 LIM zinc-binding domains. |
| Molecular Weight | 68 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in pervandate treated and untreated Huvec cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected Paxillin on 10 µg of Pervandate treated and untreated Huvec cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C for up to one year or 2-8°C for short term use from date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |