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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, DB, PIA, ChIP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-145 |
| Description | Anti-phospho-Histone H3 (Ser28) Antibody |
| Alternate Names |
|
| Background Information | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-phospho-Histone H3 (Ser28) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, DB, PIA, ChIP to detect phospho-Histone H3 (Ser28) also known as H3F3, H3F3B, H3S28P. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A 1:2,000 dilution from a representative lot detected phospho-Histone H3 (Ser28) in 10 µg of untreated and colcemid treated HeLa acid extract. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho-Histone H3 (Ser28) in HeLa cells. Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-Histone H3 (Ser28) in colcecmid treated HeLa cell lysate. DOT Blot Specificity Analysis: A representative lot of this antibody detected peptides corresponding to phospho-Histone H3 (Ser28), but not peptides corresponding to other unmodified and modifed Histones. Peptide Inhibition Analysis: A 1:500 dilution form a representative was blocked by phospho-Histone H3 (Ser28) peptides, but not non-specific peptides. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser28. |
| Epitope | Histone H3 phosphorylated at Ser28 |
| Host | Rabbit |
| Specificity | This antibody recognizes Histone H3 phosphorylated at Ser28. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Mouse. This antibody is expected to demonstrate broad species reactivity due to sequence conservation. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. Chromosome. |
| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: A 1:2,000 dilution of this antibody detected phospho-Histone H3 (Ser28) in 10 µg of HeLa acid extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |