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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H, Ch, Yeast (S. cerevisiae) | WB, ChIP | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-146 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Histone H2A (acidic patch) Antibody |
| Alternate Names |
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| Background Information | Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the beads on a string structure. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB. |
| Key Applications |
|
| Application Notes | Immunofluorescence: Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Histone H2A, Mr 14 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, rat, yeast and chicken. Broad species cross-reactivity is expected. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q16777 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 129 amino acids; 13988 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: The chromatin-associated form is phosphorylated on Thr-121 during mitosis (Probable). & Deiminated on Arg-4 in granulocytes upon calcium entry. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of Lys-27 of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. & Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. & Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). SIMILARITY: SwissProt: Q16777 ## Belongs to the histone H2A family. |
| Molecular Weight | 14 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot on acid extracts from HeLa cell lysate. Western Blot Analysis: 1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |