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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IHC, ICC, ELISA, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1533 |
| Description | Anti-JMJD3 (NT) Antibody |
| Alternate Names |
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| Background Information | JMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified polyclonal antibody supplied in PBS with 0.09% (w/v) sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3. |
| Key Applications |
|
| Application Notes | ELISA: This antibody is reported by an outside laboratory to be suitable for ELISA. Immunohistochemistry: This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry. Immunocytochemistry Analysis: Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Western Blot Analysis: The antibody recognizes JMJD3 in HeLa cell lysates. |
| Biological Information | |
|---|---|
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3. |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes the N-terminal region of human JMJD3. Additional lower MW bands are detected in some lysates that may be degradation products. |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Not tested in other species. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein g Chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: Histone demethylase that specifically demethylates Lys-27 of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 Lys-27. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Ref.5 Ref.6 Cofactor Ascorbate By similarity. Fe(2+). Ref.5 SUBUNIT STRUCTURE: Interacts with TLE1 By similarity. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and JMJD3. SUBCELLULAR lOCATION: NucleusProbable. INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Ref.4 SIMILARITY: Belongs to the UTX family. Contains 1 JmjC domain. |
| Molecular Weight | ~180 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunocytochemisty: Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 400 µL |