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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | EMSA, WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-178 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Hox A9 Antibody |
| Background Information | HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa-9 markedly expands hematopoietic stem cells. HOXA9 expression changes dramatically with age - a uniformly low level of expression during early adulthood is replaced by a frequently very high expression in adults over sixty. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A purified immunoglobulin presented in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Hox A9 with Anti-Hox A9 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in EMSA, WB, ICC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 194-272 of mouse Hox A9 |
| Host | Rabbit |
| Specificity | Hox A9 |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Based on a BLAST search, the antibody will likely cross react with Newt, Coelacanth, Dog, Chicken, and Rat Hox A9; Antelope, Mouse and Guinea Pig Hox 1.7; Frog, human and Zebrafish Hox A-9B; and Kenyan Clawed Frog Hox B.1 |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. |
| Gene Symbol |
|
| Purification Method | Protein A chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P31269 # Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. SIZE: 272 amino acids; 30172 Da SUBCELLULAR LOCATION: Nucleus. DISEASE: SwissProt: P31269 # A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98. & A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2. SIMILARITY: SwissProt: P31269 ## Belongs to the Abd-B homeobox family. & Contains 1 homeobox DNA-binding domain. |
| Molecular Weight | 36kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on whole cell lysates of Hoxa9 immortalized HF1 myeloid progenitor cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |