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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ChIP | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1799 |
| Description | Anti-NCoA-2 Antibody |
| Alternate Names |
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| Background Information | NCoA2 (nuclear receptor coactivator 2) is a transcriptional coactivator for steroid receptors and nuclear receptors. Also known as glucocorticoid receptor interacting protein 1 (GRIP1), steroid receptor coactivator-2 (SRC-2), or transcriptional mediators/intermediary factor 2 (TIF2), this coregulatory protein contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation: A representative lot of this antibody was used for chIP. See Monroe, D.g., et Al. (2006) J. Biol. Chem. 281(39):28596-28604. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human NCoA-2 |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes NCoA-2. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with human. Homology to mouse and rat is 95%. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. SUBUNIT STRUCTURE: Present in a complex containing CARM1 and EP300/P300, and interacts with CARM1 and NR3C2 By similarity. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with ESR1, RARA and RXRA. SUBCELLULAR LOCATION: Nucleus. DOMAIN: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. Ref.4 The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding. Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. INVOLVEMENT IN DISEASE: Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv8(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation. SEQUENCE SIMILARITIES: Belongs to the SRC/p160 nuclear receptor coactivator family. Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 PAS (PER-ARNT-SIM) domain. |
| Molecular Weight | ~ 160 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in U2 OS cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected SRC2 on 10 µg of U2 OS cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |