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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-324 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PP2A Antibody, C subunit |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/mL BSA before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PP2A Antibody, C subunit is validated for use in WB for the detection of PP2A. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide corresponding to amino acids 290-304 (DPAPRRGEPHVTRRT) of human PP2A |
| Host | Rabbit |
| Specificity | PP2A catalytic subunit |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. |
| Gene Symbol |
|
| Purification Method | Immunoaffinity Chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity). SIZE: 309 amino acids; 35594 Da SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B/PR72/PR130/PR59 and R5/B/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. SUBCELLULAR LOCATION: Cytoplasm. PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily. |
| Molecular Weight | 36kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from mouse brain, heart, testes and spleen homogenates |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |