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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-326 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-Cofilin 2 (Ser3) Antibody |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-Cofilin 2 (Ser3) Antibody detects level of phospho-Cofilin 2 (Ser3) & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide corresponding to amino acids 1-10 (MA[PS]GVTVNDE-C) of human phospho-Cofilin 2 |
| Host | Rabbit |
| Specificity | phospho-Cofilin 2 |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric g-actin in a pH-dependent manner. (Gillett et Al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et Al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et Al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM] |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Protein A chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9Y281 # Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind g- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods (By similarity). SIZE: 166 amino acids; 18737 Da SUBCELLULAR LOCATION: Nucleus matrix (By similarity). Cytoplasm, cytoskeleton (By similarity). TISSUE SPECIFICITY: Isoform CFL2b is expressed predominantly in skeletal muscle and heart, while isoform CFL2a is expressed in various tissues. PTM: The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. DISEASE: SwissProt: Q9Y281 # Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. SIMILARITY: SwissProt: Q9Y281 ## Belongs to the actin-binding proteins ADF family. & Contains 1 ADF-H domain. |
| Molecular Weight | 21kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from L6 cells, treated with LIM Kinase |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |