Name: Millipore Anti-Rest Antibody
SKU: MILL-07-579
Price: 590.52 USD

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Purified	Polyclonal Antibody

Description
Catalogue Number	07-579
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-REST Antibody
Alternate Names	Neural-restrictive silencer factor RE1-silencing transcription factor X2 box repressor neuron restrictive silencer factor repressor binding to the X2 box
Background Information	RE1-silencing transcription factor (UniProt: Q13127; also known as REST, Neural-restrictive silencer factor, X2 box repressor) is encoded by the REST (also known as NRSF, XBR) gene (Gene ID: 5978) in human. REST is a transcriptional repressor that acts by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE). It is ubiquitously expressed, and higher expression is found in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes, and ovary. It is also found in undifferentiated neuronal progenitor cells and is believed to acts as a master negative regular of neurogenesis. Expression of REST is reported to be cell cycle-dependent with reduced levels in the G2 phase. It is involved in maintaining the quiescent state of adult neural stem cells and prevents their premature differentiation into mature neurons. REST restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. REST also mediates repression by recruiting the BHC complex at RE1/NRSE sites, which acts by deacetylating and demethylating specific sites on histones and acting as a chromatin modifier. Mutations in REST gene can lead to Wilms tumors, a pediatric malignancy of the kidney that occurs due to uncontrolled multiplication of renal stem, stromal, and epithelial cells. (Ref.: Mahamdallie, SS., et Al. (2015). Nat. Genet. 47(12); 1471-1474; Huang, Z., et Al. (2011). Nat. Cell Biol. 13(2); 142-152).

Product Information
Format	Purified
Control	HeLa nuclear extract Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl with 0.07% sodium azide with 30% glycerol.
Quality Level	MQ100

Applications
Application	Anti-REST, Cat. No. 07-579, is a rabbit polyclonal antibody that detects REST and is tested for use in Western Blotting.
Key Applications	Western Blotting
Application Notes	Updated information-Rest is glycosylated. From http://amsdottorato.cib.unibo.it/687/ From the summary: "Finally, the glycosylation pattern of the REST protein was analysed, moving from the observation that the molecular weight calculated on REST sequence is about 116 kDa but using western blotting this transcription factor appears to have distinct apparent molecular weight (see Table 1.1): this difference could be explained by post-translational modifications of the proteins, like glycosylation. In fact recently, several studies underlined the importance of O-glycosylation in modulating transcriptional silencing, protein phosphorylation, protein degradation by proteasome and protein–protein interactions [Julenius et Al., 2005; Zachara and Hart, 2006]. Deglycosilating analysis showed that REST protein in SH-SY5Y and HEK293 cells is Oglycosylated and not N-glycosylated. Moreover, using several combination of deglycosilating enzymes it is possible to hypothesize the presence of Gal-β(1-3)-GalNAc residues on the endogenous REST, while β(1-4)-linked galactose residues may be present on recombinant REST protein expressed in HEK293 cells. However, the O-glycosylation process produces an immense multiplicity of chemical structures and monosaccharides must be sequentially hydrolyzed by a series of exoglycosidase. Further experiments are needed to characterize all the post-translational modification of the transcription factor REST." In particular the authors show that REST migrates at 200kd in SH-SY5Y cells.

Biological Information
Immunogen	GST-tagged recombinant fragment corresponding to 297 amino acids from the C-terminal region of human RE1-silencing transcription factor (REST).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This Rabbit polyclonal antibody detects RE1-silencing transcription factor (REST). It targets an epitope within the C-terminal region.
Isotype	IgG
Species Reactivity	Human
Species Reactivity Note	Human.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_005612
Entrez Gene Summary	This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.
Gene Symbol	REST NRSF XBR
Purification Method	Protein A Purfied
UniProt Number	Q13127
UniProt Summary	FUNCTION: SwissProt: Q13127 # Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. SIZE: 1097 amino acids; 121888 Da SUBUNIT: Interacts with SIN3A, SIN3B and RCOR1. SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. SIMILARITY: SwissProt: Q13127 ## Contains 9 C2H2-type zinc fingers.
Molecular Weight	~160 kDa observed; 121.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected REST in HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	200 µg

SKU	MILL-07-579
Supplier Part Number	07-579
UM	EA
UNSPSC	12352203
Manufacturer	Millipore
ProductLine	Mill
Qty	1
MinOrderQty	1
Weight	10.000000
Lead Time	10
Hazardous	N
Energy Star	No
Green	No
Controlled	N

NETA PART NO.	Quantity

Millipore Anti-Rest Antibody

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Key Specifications Table

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