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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-618 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-SF-1 Antibody |
| Background Information | Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, SF-1 localizes to theca/interstitial cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SF-1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of SF-1 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | DNA binding domain of SF-1 from mouse, produced in E. coli as a GST fusion protein and partially purified via GST-agarose column chromatography |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes SF-1. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q13285 # Transcriptional activator. Seems to be essential for sexual differentiation and sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes. 5- YCAAGGYC-3 and 5-RRAGGTCA-3 are the consensus sequences for the recognition by NR5A1/FTZF1. The SFPQ-NONO-NR5A1/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. SIZE: 461 amino acids; 51636 Da SUBUNIT: Binds DNA as a monomer. Interacts with NR0B2 (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1/SF-1. Interacts with NCOA2. SUBCELLULAR LOCATION: Nucleus. PTM: Acetylation stimulates the transcriptional activity. DISEASE: SwissProt: Q13285 # Defects in NR5A1 are a cause of XY sex reversal with adrenal failure [MIM:184757]. This disease is characterized by normal female external genitalia and retention of the uterus. & Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect [MIM:184757]. The disease is characterized by severe slackness, muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH. SIMILARITY: SwissProt: Q13285 ## Belongs to the nuclear hormone receptor family. NR5 subfamily. & Contains 1 nuclear receptor DNA-binding domain. |
| Molecular Weight | 53 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on RIPA lysates from Y1 cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |