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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-706 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-WDR5 Antibody |
| Alternate Names |
|
| Background Information | WD repeat-containing protein 5 (also known as BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, WD-repeat protein 5, Set1c WD40 repeat protein homolog, cilia and flagella associated protein 89) is a nuclear protein encoded by the WDR5 (also known as BIG3, CFAP89, SWD3) gene in human. This protein contains 7 WD (Trp-Asp) repeats and belongs to the WD repeat protein family whose members are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeat-containing protein 5 is a component of several complexes that regulate histone methylation and acetylation levels, including ATAC (ADA2A-containing complex) complex, SET1 complex, MLL1/MLL, MLL2/3 (also named ASCOM complex), MLL4/WBP7, and NSL (non-specific lethal) complex. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect WDR5 using this Anti-WDR5 Antibody validated for use in WB. |
| Key Applications |
|
| Application Notes | Western Blot: 0.05-0.25 μg/mL using HeLa nuclear extracts. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of human WDR5. |
| Epitope | Near N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes WDR5, MW 36 kDa. A related protein band of 43 kDa that was blocked by the immunizing peptide was also detected. Identification of WDR5 protein at 36 kDa was confirmed with siRNA knockdown by an independent laboratory. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~39 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |