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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC | Rb | AlexaFluor®555 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-748-AF555 |
| Description | Anti-monomethyl Histone H4 (Lys20), Alexa Fluor® 555 conjugate |
| Alternate Names |
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| Background Information | Histone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression. |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Presentation | Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-monomethyl Histone H4 (Lys20), Alexa Fluor® 555 conjugate is an antibody against monomethyl Histone H4 (Lys20), Alexa Fluor® 555 conjugate for use in Immunocytochemistry. |
| Key Applications |
|
| Application Notes | The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[Kme]VLRDN) of human Histone H4 with monomethylated Lys20. |
| Epitope | H4K20me1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects histone H4 with monomethylated Lys20 (H4K20me1). |
| Species Reactivity |
|
| Species Reactivity Note | Human. Broad species cross-reactivity expected due to sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~10 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |