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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ELISA | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-796 |
| Description | Anti-PADI4 Antibody, CT |
| Alternate Names |
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| Background Information | PADI4 (Protein-arginine deiminase type-4) is a member of a family of enzymes responsible for the conversion of arginine residues to citrulline residues via catalysis of the deimination of the arginine residues. PADI4 down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. This protein may play a role in granulocyte and macrophage development leading to inflammation and immune response. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PADI4 Antibody, C-terminus is an antibody against PADI4 for use in WB, ELISA. |
| Key Applications |
|
| Application Notes | ELISA: A 1:1000 dilution of a previous lot was used on ELISA. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic linear peptide corresponding to the C-terminal region of PADI4. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes PADI4 at and around the C-terminus. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt Number |
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| UniProt Summary | FUNCTION: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at Arg-8 and/or Arg-17 and histone H4 at Arg-3, which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at Arg-2142, which favors its interaction with NCOA2/GRIP1. CATALYTIC ACTIVITY: Protein L-arginine + H2O = protein L-citrulline + NH3. COFACTOR: Binds 5 calcium ions per subunit. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasmic granule. Note= Cytoplasmic granules of eosinophils and neutrophils. TISSUE SPECIFICITY: Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. INVOLVEMENT IN DISEASE: PADI4 is thought to be a rheumatoid arthritis susceptibility locus [MIM:180300]. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. SEQUENCE SIMILARITIES: Belongs to the protein arginine deiminase family. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.055 mM for fibrinogen KM=0.064 mM for filaggrin Vmax=33.2 µmol/h/mg enzyme toward fibrinogen Vmax=8.0 µmol/h/mg enzyme toward filaggrin pH dependence: Optimum pH is 6.5-9.0. |
| Molecular Weight | ~74 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on HEK293 cell lysate. Western Blotting Analysis: 1:25-1:500 dilution of this antibody detected PADI4 in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |