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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, ChIP | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-820 |
| Description | Anti-Klf2 Antibody |
| Alternate Names |
|
| Background Information | Krueppel-like factor 2 (Klf2) is a novel protein belonging to the KLF family of transcription factors, a subfamily of approximately 20 members characterized by their possession of a zinc-finger domain and functioning as transcriptional regulators for cellular differentiation and growth. Klf2 activates transcription through its binding of the beta-globin gene promoter CACCC box. Studies have shown Klf2 to have critical roles in both the function and differentiation of several cell types including; adipocytes, lung cells, T lymphocytes, and monocytes. It has also been newly identified as an endothelial transcription regulator for pro-inflammatory activation, and is a requirement for the migration of smooth muscle cells and normal vessel formation. Shear stress and pro-inflammatory cytokines are considered to be potent inhibitors of Klf2. Expression is primarily observed in lung and spleen tissue, but it has also been found in aortic tissue. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Klf2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Klf2 also known as Kruppel-like factor 2 (lung), Lung krueppel-like factor. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Klf2 from 2 x 10E6 to 1 x 10E7 mouse embryonic stem cells. (Huck Ng, Genome Institute of Singapore.) |
| Biological Information | |
|---|---|
| Immunogen | GST-fusion protein with histidine-tagged mouse Klf2. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Binds to the CACCC box in the beta-globin gene promoter and activates transcription. SUBUNIT STRUCTURE: Interacts with WWP1 (By similarity). SUBCELLULAR LOCATION: Nucleus (By similarity). TISSUE SPECIFICTY: Predominant expression in the lungs and spleen. PTM: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. SEQUENCE SIMILARITIES: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. |
| Molecular Weight | ~40 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse embryonic stem cell chromatin extract. Western Blot Analysis: A 1:1,000 dilution of this antibody detected Klf2 in 10 µg of mouse embryonic stem cell chromatin extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |