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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IP, ChIP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-823 |
| Description | Anti-JMJD1A Antibody |
| Alternate Names |
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| Background Information | JMJD1A (jumonji domain-containing 1a) is a histone demethylase that plays a key role in the histone code. Functionally, this protein is known to demethylate mono- and dimethylated histoneH3 at lysine 9, while demonstrating virtually no demethylation activity for trimethylated lysine 9 of histone H3. A product of the JHDM2A gene, this protein has been demonstrated to play a role in hypoxia, spermatogenesis, and cell differentiation with loss of function of JMJD1A resulting embryonic stem cell differentiation. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-JMJD1A Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect JMJD1A also known as lysine (K)-specific demethylase 3A, testis-specific protein A. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 5 µg of a representative lot of this antibody immunoprecipitated JMJD1A in HeLa nuclear extract. Western Blot Analysis: A representitive lot of this antibody detected JMJD1A in mouse ESC chromatin extract. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse JMJD1A. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Summary: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 Lys-9 residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 Lys-9. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 Lys-9 demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. COFACTOR: Binds 1 Fe2+ ion per subunit. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note: Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity). DOMAIN: The JmjC domain and the C6-type zinc-finger are required for the demethylation activity. Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors (By similarity). SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family. Contains 1 JmjC domain. SEQUENCE CAUTION: The sequence BAA34462.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
| Molecular Weight | ~147 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 nuclear extract. Western Blot Analysis: 1 µg/mL of this antibody detected JMJD1A on 10 µg of HEK293 nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |