Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Yeast (S. cerevisiae) | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-874 |
| Description | Anti-SAP30 (yeast) Antibody |
| Alternate Names |
|
| Background Information | SAP30 (Sin3A-associated protein) is a widely expressed nuclear protein that is functionally conserved between human and yeast. It is a component of the multiprotein histone deacetylase complex containing the transcriptional repressor Sin3, multiple histone deacetylases (HDAC1 and HDAC2), histone binding proteins (RbAp46 and RbAp48) and other polypeptides. SAP30 can recruit the deacetylase complex to promoter DNA in a Sin3 independent manner and is involved in the recruitment of the mSin3 complex to a specific subset of N-CoR corepressor complexes. The latter function suggests a role for SAP30 in N-CoR-mediated repression. Furthermore, SAP30 may be involved in imparting target specificity to the deacetylase complex. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A purified IgG in 0.1M Tris-Glycine (pH7.4) 150 mm NaCl with 0.05% NaN3. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-SAP30 (yeast) Antibody is a Rabbit Polyclonal Antibody for detection of SAP30 (yeast) also known as Sin3 corepressor complex subunit SAP30 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Immunogen was a synthetic peptide corresponding to a.a. 141-157 of yeast SAP30, conjugated to KLH. |
| Epitope | a.a. 141-157 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes yeast SAP30 at ~27 kDa. |
| Species Reactivity |
|
| Species Reactivity Note | Yeast (Saccharomyces cerevisiae). Not tested in other species. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. SUBUNIT:Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. SUBCELLULAR LOCATION: Nucleus. MISCELLANEOUS: Present with 704 molecules/cell in log phase SD medium. SIMILARITY: Belongs to the SAP30 family. |
| Molecular Weight | ~27 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: 1 μg/mL of this antibody detected SAP30 in a yeast extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |