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Millipore Normal Rabbit Igg Polyclonal Antibody Control Validated For Use In Immunoprecipitation & Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Immunoglobulin g (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
Format
Unconjugated
Presentation
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at –20°C.
Quality Level
MQ100
Applications
Application
Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.
Key Applications
Immunoprecipitation
Western Blotting
Biological Information
Epitope
N/A
Concentration
1 mg/mL
Host
Rabbit
Isotype
IgG
Antibody Type
Polyclonal Antibody
Purification Method
Purified
Product Usage Statements
Quality Assurance
Routinely evaluated by IP/WB as a non-specific IgG control.
Immunoprecipitation/Western Blot Assay: 2 µg of this lot was used as a non-specific IgG control in an immunoprecipitation / western blot assay.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product centrifuge the original vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
