| Description | |
|---|---|
| Catalogue Number | 12-373 |
| Brand Family | Upstate |
| Trade Name |
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| Description | CHKtide (CHK1/CHK2 substrate peptide) |
| Product Information | |
|---|---|
| Presentation | Lyophilized powder |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | CHKtide (CHK1/CHK2 substrate peptide) primarily used in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Entrez Gene Number |
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| Entrez Gene Summary | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| Protein Target | CHK |
| Target Sub-Family | CAMK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on Ser-216, preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at Thr-18 and Ser-20. COFACTOR: Magnesium. SIZE: 543 amino acids; 60915 Da SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus. TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]. SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Protein Kinase Assay as a substrate for the active CHK1 (Catalog #14-346) and CHK2 (Catalog #14-347) kinases. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 1 mg |