Key Specifications Table
| Key Applications | Entrez Gene Number | Uni Prot Number |
|---|---|---|
| IP, APA | NM_175055.2 | Q8N257 |
| Description | |
|---|---|
| Catalogue Number | 12-408 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Histone H2B Peptide, biotin conjugate, residues 21-41 |
| Product Information | |
|---|---|
| Presentation | Lyophilized powder |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. |
| Key Applications |
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| Biological Information | |
|---|---|
| Entrez Gene Number |
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| Entrez Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 126 amino acids; 13908 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 Lys-4 and Lys-79 methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination. SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated as a substrate for histone-modifying enzymes. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |