| Description | |
|---|---|
| Catalogue Number | 12-549 |
| Brand Family | Upstate |
| Trade Name |
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| Description | STK Substrate 2 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | STK Substrate 2 primarily used in Kinase Assays. |
| Key Applications |
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| Biological Information | |
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| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. |
| Gene Symbol |
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| Protein Target | Akt3/PKBγ |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9Y243 # IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. SIZE: 479 amino acids; 55775 Da SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. TISSUE SPECIFICITY: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. DOMAIN:SwissProt: Q9Y243 Binding of the pH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 pH domain. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely used as a serine/threonine kinase substrate with KinEASE™ fluorescence polarization assays |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | stable for 6 months at -20°C from date of shipment |
| Packaging Information | |
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| Material Size | 120 µL |