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Millipore Active, N-Terminal Gst-Tagged & C-Terminal 6his Tagged, Recombinant, Human Wee1, Amino Acids 214-End. For Use In Kinase Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on Tyr-15. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on Tyr-15 and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source
N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_003390
Gene Symbol
WEE1
Protein Target
WEE1
Purification Method
Purified using glutathione agarose.
Target Sub-Family
CK1
UniProt Number
P30291
Molecular Weight
77 kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
6 months at -70°C
Packaging Information
Material Size
10 µg
Material Package
Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
