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Millipore Anti-Phosphotyrosine Antibody, Clone 4g10®, Hrp Conjugate Detects Level Of Phosphotyrosine & Has Been Published & Validated For Use In Elisa & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
Background Information
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10®. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Product Information
Format
HRP
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Presentation
200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% Kathon® . Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
Quality Level
MQ100
Applications
Application
Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in ELISA & WB.
Key Applications
ELISA
Western Blotting
Application Notes
For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
Biological Information
Immunogen
Phosphotyramine-KLH.
Epitope
N/A
Clone
4G10®
Host
Mouse
Specificity
Recognizes tyrosine-phosphorylated proteins from all species.
Isotype
IgG2bκ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Modifications
Phosphorylation
Purification Method
Protein G-Sepharose™ Chromatography
Molecular Weight
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Quality Assurance
Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis: Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap. NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
